The cohort consists of 534 healthy individuals, of whom 44% are male and 56% are female, all of Western-European genetic background.

The inclusion of the volunteers took place between 08/2013 until 12/2014 in the Radboud University Medical Center, the Netherlands. The age of the cohort ranges from 18 to 75, with the majority (421 individuals) being 30 years old or younger.

Within this cohort;

– Immune cell counts
– Cytokine production upon incubation with various viral, fungal and bacterial stimuli
– Immunoglobulins
– Gut microbiome
– SNP genotyping
– Transcriptome (from a subset of the cohort)
– Metabolome

measurements are available.

*The 500FG study was approved by the Ethical Committee of Radboud University Nijmegen (NL42561.091.12, 2012/550).

Selected papers

Chu, X., Jaeger, M., Beumer, J., Bakker, O. B., Aguirre-Gamboa, R., Oosting, M., Smeekens, S. P., Moorlag, S., Mourits, V. P., Koeken, V. A. C. M., de Bree, C., Jansen, T., Mathews, I. T., Dao, K., Najhawan, M., Watrous, J. D., Joosten, I., Sharma, S., Koenen, H. J. P. M., Withoff, S., … Li, Y. (2021). Integration of metabolomics, genomics, and immune phenotypes reveals the causal roles of metabolites in disease. Genome biology, 22(1), 198. https://doi.org/10.1186/s13059-021-02413-z

Schirmer, M., Smeekens, S. P., Vlamakis, H., Jaeger, M., Oosting, M., Franzosa, E. A., Ter Horst, R., Jansen, T., Jacobs, L., Bonder, M. J., Kurilshikov, A., Fu, J., Joosten, L. A. B., Zhernakova, A., Huttenhower, C., Wijmenga, C., Netea, M. G., & Xavier, R. J. (2016). Linking the Human Gut Microbiome to Inflammatory Cytokine Production Capacity. Cell, 167(4), 1125–1136.e8. https://doi.org/10.1016/j.cell.2016.10.020

Li, Y., Oosting, M., Smeekens, S. P., Jaeger, M., Aguirre-Gamboa, R., Le, K. T. T., Deelen, P., Ricaño-Ponce, I., Schoffelen, T., Jansen, A. F. M., Swertz, M. A., Withoff, S., van de Vosse, E., van Deuren, M., van de Veerdonk, F., Zhernakova, A., van der Meer, J. W. M., Xavier, R. J., Franke, L., Joosten, L. A. B., … Netea, M. G. (2016). A Functional Genomics Approach to Understand Variation in Cytokine Production in Humans. Cell, 167(4), 1099–1110.e14. https://doi.org/10.1016/j.cell.2016.10.017

Ter Horst, R., Jaeger, M., Smeekens, S. P., Oosting, M., Swertz, M. A., Li, Y., Kumar, V., Diavatopoulos, D. A., Jansen, A. F. M., Lemmers, H., Toenhake-Dijkstra, H., van Herwaarden, A. E., Janssen, M., van der Molen, R. G., Joosten, I., Sweep, F. C. G. J., Smit, J. W., Netea-Maier, R. T., Koenders, M. M. J. F., Xavier, R. J., … Netea, M. G. (2016). Host and Environmental Factors Influencing Individual Human Cytokine Responses. Cell, 167(4), 1111–1124.e13. https://doi.org/10.1016/j.cell.2016.10.018