300DM

This cohort included 243 individuals of Western European ancestry with type 1 diabetes, ranging in age from 20 to 84 years.

Study participants, of whom 46% are female, were enrolled between February 2016 and June 2017 in the Radboud University Medical Center. After written informed consent was obtained, venous blood samples were collected in EDTA tubes from all participants, who presented in a non-fasting state, between 8:00 and 11:00 a.m.

In this cohort, the available data are as follows:

• – Demographics and health- and disease-related information
• – Immunophenotyping
• – Cytokine production upon incubation with various stimuli
• – Gut microbiome
• – Proteomics
• – Genotyping

*The study was approved by the ethical committee of Radboud University Nijmegen (NL.54214.091.15).

Selected Papers

van Heck, J. I. P., Ajie, M., Joosten, L. A. B., Tack, C. J., & Stienstra, R. (2025). Circulating inflammatory proteins are elevated in type 1 and type 2 diabetes and associated to complications. Diabetes, obesity & metabolism, 27(2), 719–728. https://doi.org/10.1111/dom.16066

He, X., Wang, X., van Heck, J., van Cranenbroek, B., van Rijssen, E., Stienstra, R., Netea, M. G., Joosten, I., Tack, C. J., & Koenen, H. J. P. M. (2024). Blood immune cell profiling in adults with longstanding type 1 diabetes is associated with macrovascular complications. Frontiers in immunology, 15, 1401542. https://doi.org/10.3389/fimmu.2024.1401542

Ajie, M., van Heck, J. I. P., Janssen, A. W. M., Meijer, R. I., Tack, C. J., & Stienstra, R. (2023). Disease Duration and Chronic Complications Associate With Immune Activation in Individuals With Longstanding Type 1 Diabetes. The Journal of clinical endocrinology and metabolism, 108(8), 1909–1920. https://doi.org/10.1210/clinem/dgad087

van Heck, J. I. P., Gacesa, R., Stienstra, R., Fu, J., Zhernakova, A., Harmsen, H. J. M., Weersma, R. K., Joosten, L. A. B., & Tack, C. J. (2022). The Gut Microbiome Composition Is Altered in Long-standing Type 1 Diabetes and Associates With Glycemic Control and Disease-Related Complications. Diabetes care, 45(9), 2084–2094. https://doi.org/10.2337/dc21-2225

Chu, X., Janssen, A. W. M., Koenen, H., Chang, L., He, X., Joosten, I., Stienstra, R., Kuijpers, Y., Wijmenga, C., Xu, C. J., Netea, M. G., Tack, C. J., & Li, Y. (2022). A genome-wide functional genomics approach uncovers genetic determinants of immune phenotypes in type 1 diabetes. eLife, 11, e73709. https://doi.org/10.7554/eLife.73709

Janssen, A. W. M., Stienstra, R., Jaeger, M., van Gool, A. J., Joosten, L. A. B., Netea, M. G., Riksen, N. P., & Tack, C. J. (2021). Understanding the increased risk of infections in diabetes: innate and adaptive immune responses in type 1 diabetes. Metabolism: clinical and experimental, 121, 154795. https://doi.org/10.1016/j.metabol.2021.154795