2000HIV
Posted on 8 January 2026
This study consists of 1895 participants. The majority of participants were cis-male, and of white ethnical ancestry.
Asymptomatic people living with HIV without signs or symptoms of an acute condition were included between 2019 and 2021, originating from the following four Dutch HIV treatment centers: Erasmus MC Rotterdam, OLVG Amsterdam, Tweesteden Ziekenhuis Tilburg, Radboudumc Nijmegen.
Withing this cohort;
- – Questionnaires regarding health
- – Clinical data such as CD4 nadir, viral load
- – SNP genotyping
- – DNA methylation
- – Microbiome
- – Immune cell profiling
- – Cytokine production upon incubation with various viral, fungal and bacterial stimuli
- – Proteomics
- – Transcriptomics
measurements are available.
*The 2000HIV study was approved by the Medical Ethical Review Committee Oost Nederland, Nijmegen, the Netherlands NL68056.091.81 and published at clinicaltrials.gov (2000HIV study – NTC03994835).
Selected Publications
Vadaq, N., Groenendijk, A. L., Dos Santos, J. C., Mehta, K., Wit, F. W. N. M., Vos, W. A. J. W., Blaauw, M. J. T., van Eekeren, L. E., Lambrechts, L., Rutsaert, S., Nelwan, E. J., Xu, C. J., Joosten, L. A. B., de Mast, Q., Matzaraki, V., van Lunzen, J., Rokx, C., Verbon, A., Netea, M. G., Vandekerckhove, L., … van der Ven, A. J. A. M. (2026). Longitudinal plasma proteomic signatures of elite and viremic spontaneous HIV controllers. Nature communications, 17(1), 1173. https://doi.org/10.1038/s41467-025-67939-3
Navas, A., Dos Santos, J. C., van Cranenbroek, B., Vadaq, N., Groenendijk, A. L., Vos, W. A. J. W., Blaauw, M. J. T., van Eekeren, L., Rokx, C., Stalenhoef, J. E., Berrevoets, M. A. H., Netea, M. G., Joosten, L. A. B., van der Ven, A. J. A. M., & Koenen, H. J. P. M. (2025). Circulating immune landscape and immune signatures in spontaneous HIV controllers. Frontiers in immunology, 16, 1642482. https://doi.org/10.3389/fimmu.2025.1642482
Gupta, M. K., Cristina Dos Santos, J., Vazquez, V. R., Ruijten, S. D. E., Navas, A., Jiang, X., Liu, Z., Zhang, Z., Botey-Bataller, J., Singh, O., Vadaq, N., Groenendijk, A. L., Vos, W. A. J. W., Blaauw, M. J. T., van Eekeren, L. E., Li, Y., Joosten, L. A. B., Rokx, C., Verbon, A., Matzaraki, V., … Xu, C. J. (2025). DNA methylation landscapes of HIV controllers: an epigenome-wide association study. EBioMedicine, 121, 105999. https://doi.org/10.1016/j.ebiom.2025.105999
Navas, A., Matzaraki, V., van Eekeren, L. E., Blaauw, M. J. T., Groenendijk, A. L., Vos, W. A. J. W., Jacobs-Cleophas, M., Dos Santos, J. C., van der Ven, A. J. A. M., Joosten, L. A. B., & Netea, M. G. (2025). Plasma Proteomic Signature as a Predictor of Age Advancement in People Living With HIV. Aging cell, 24(5), e14468. https://doi.org/10.1111/acel.14468
Otten, T., Jiang, X., Gupta, M. K., Vadaq, N., Cleophas-Jacobs, M., Dos Santos, J. C., Groenendijk, A., Vos, W., van Eekeren, L. E., Blaauw, M. J. T., Meeder, E. M. G., Richel, O., Matzaraki, V., van Lunzen, J., Joosten, L. A. B., Li, Y., Xu, C. J., van der Ven, A., & Netea, M. G. (2024). Impact of COVID-19, lockdowns and vaccination on immune responses in a HIV cohort in the Netherlands. Frontiers in immunology, 15, 1459593. https://doi.org/10.3389/fimmu.2024.1459593
Vos, W. A. J. W., Groenendijk, A. L., Blaauw, M. J. T., van Eekeren, L. E., Navas, A., Cleophas, M. C. P., Vadaq, N., Matzaraki, V., Dos Santos, J. C., Meeder, E. M. G., Fröberg, J., Weijers, G., Zhang, Y., Fu, J., Ter Horst, R., Bock, C., Knoll, R., Aschenbrenner, A. C., Schultze, J., Vanderkerckhove, L., … van der Ven, A. J. A. M. (2022). The 2000HIV study: Design, multi-omics methods and participant characteristics. Frontiers in immunology, 13, 982746. https://doi.org/10.3389/fimmu.2022.982746